Sentence examples for stopping buffer was added from inspiring English sources

Mixtures were maintained at 52°C for 5 min, and then 120 μL of stopping buffer was added.

As 1 μL of stop buffer was added into the medium to stop the reaction, shRNA from NPs was extracted according to the above method and the products were analyzed by agarose gel electrophoresis.

After 1 hour stop buffer was added and assayed for phospho-ATF2 in the MSD assay.

To the remaining nuclei (0.1 ml, set aside for undigested DNA control) an equal volume of 2X stop buffer was added.

As above, 200 μl of stop buffer is added and the DNA is extracted and precipitated.

Ten microlitres of cell lysate was then added to 50 μl of luciferase substrate solution, following which 50 μl of stop & glow buffer was added for Renilla reading.

An equal volume of 2× SDS sample buffer was added to stop the reaction and the samples were boiled for 10 minutes.

At each time point, 4 × SDS loading buffer was added to stop the cleavage.

Then 20 ml of PBS buffer was added to stop lysis reaction.

After 15 min of incubation at room temperature, 50 μL of loading buffer was added to stop the reaction.

Reactions were incubated for 10 min at 30°C, sample buffer was added to stop the reaction and 50 μl of each sample were analyzed by 10% SDS-PAGE.