Sentence examples for stopping buffer from inspiring English sources

This mixture was incubated at 37°C for 2 hours and stopped by adding 5 μl stopping buffer.

Mixtures were maintained at 52°C for 5 min, and then 120 μL of stopping buffer was added.

The reaction was stopped by the addition of 0.5 ml of stopping buffer (6 M guanidine-HCl, and 1 mM DNTB).

The reaction was terminated by adding 5  μL of stopping buffer (10% SDS, 25 mM EDTA, 0.025% bromophenol blue, and 40% glycerol).

The reaction incubation was carried out at 37°C in 75 mM potassium phosphate, pH 7.4 buffer, and stopped by stopping buffer as described [ 30, 31].

Following incubation at 37°C for 30 min, the reaction was terminated by adding 5 μl of stopping buffer (final concentration: 1% sodium dodecyl sulfate (SDS), 15% glycerol, 0.5% Bromophenol blue, and 50 mM EDTA pH 8).

Make sure that stopping buffers are covered in soft materials to cushion impact.

After 30 min vibration, with 1× substrate and stop buffer each prepared, luciferase activity was measured by Ascent software on chemiluminescent plate reader (Thermo).

Then stop buffer was applied before slides were incubated in anti-digoxigenin conjugate solution in a humidified chamber for 30 minutes at room temperature in dark.

Stop buffer is 1% SDS, 40 mm EDTA.

Cells were washed with stop buffer for 10 min and then incubated with anti-digoxigenin conjugate for 30 min at 25 °C.