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"What are you writing?" we asked when she stopped to insert another page.
Drilling may be stopped for logging and testing (see below Well logging and drill stem testing), and it also may be stopped to insert casing and cement it to the outer circumference of the borehole.
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His ammunition spent, the assailant stopped to reload, the authorities said, inserting a 31-round clip into the chamber of his Glock semiautomatic pistol before raising the gun again.
In Bacillus subtilis UGA codons are used to terminate mRNA translation (stop codons) and to insert tryptophan, creating readthrough proteins [6].
Digestion with BspH I (overlapping the start codon) and Spe I (overlapping the stop codon) was used to insert the Pcar_1041 gene between the flanking regions of GSU1659.
To obtain the EGFP-tagged Rn specific to the E/F isoforms, we decided to conduct a second round of CRISPR-mediated editing in the Rn-EGFP line to insert stop codons at the beginning of the first C isoform-specific exon.
Podocalyxin mutants were generated by PCR from cDNA using primers designed to insert early stop codons after the juxtamembrane HQRF sequence (Δtail) or before the C-terminal DTHL (ΔDTHL).
To insert a stop codon with the recombinant virus with a flag epitope fused to N- terminus of the UL77 ORF, the RpsLneo gene was inserted into the UL76 of flagUL77 BAC DNA as described above (see figure 4, light panel).
In step 1, we aimed to insert a STOP-3xP3-dsRed cassette flanked by two attP sites using a donor plasmid.
Truncation mutants of the carboxy tail were constructed using PCR to insert a stop codon (TAG) at various locations within the mouse gene.
I also designed two overlapping sgRNAs to target the NHEJ gene, lig-4, and a 60mer oligo as the homologous recombination template to insert a stop codon (oligos were unpurified).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com