After 24 hr, fermentations were stopped and samples were taken.
After the reaction was stopped, myoglobin samples were extensively dialyzed.
Upon the completion of the trial, the model was stopped and samples were taken out, and the excess surface moisture was removed with a filter paper.
Then the model was stopped and samples were taken out, dried with a filter paper to remove surface moisture, and measured for weight W t (in grams).
To stop labeling, samples were washed/spun twice with ice cold PBS at 4 °C.
Cultures were induced for 6 hours and β-galactosidase activity was measured as described previously [ 74] with the following changes: 1) assays were performed at 37°C, and 2) after stopping reaction, samples were centrifuged and OD420 of the supernatant was measured, eliminating the OD550 correction for cell debris.
We call the new method "phase homogenization in electrochemical boriding" (PHEB), in which carbon steel samples undergo electrochemical boriding for about 15 min at 950 °C in a molten electrolyte consisting of 90% borax and 10% sodium carbonate, then after the electrical power to the electrodes is stopped, the samples are left in the bath for an additional 45 min without any polarization.
Then, heparin infusion was stopped and blood samples were obtained to perform HIT testing.
The reaction was stopped on ice, samples were centrifuged at 500×g for 10 min at 4 °C and supernatants were analyzed for cysLT levels using an enzyme immunoassay kit which detects LTC4, D4 and E4 according to the manufacturer's instructions (Sapphire Biosciences, Australia).
Iterations were stopped when enough samples were generated to see agreement between the estimates from the different chains [8].
