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Then, incubations were stopped by cell harvesting (Filtermate 196 Harvester, Packard-Canberra) and lysis: After removing the supernatant (see below), the adherent cells were released by 5 min treatment with 100 ml 0.125% trypsin in PBS at 37°C, harvested on a filterplate and washed 3 x with redistilled water.
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Trogocytosis was initiated by moving cells to a 37°C 5% CO2 incubator, and stopped by moving cell mixtures to 4°C.
As Conover stopped by his cell one day and engaged in a brief conversation about a book of antiquated anthropology that Powerful was reading, the inmate looked at the correction officer inquisitively.
But I'm gonna shoWhenm thet I'm not gonna let thistopped.
Finally, the reaction was stopped by washing cells once in PBS and, after counterstaining with propidium iodide, cells were visualized in a microscope.
The reaction was stopped by washing cells twice with 1 mL of ice-cold wash buffer, and cells were lysed in 1 mL of 0.2 M NaOH.
After the cells were washed three times with PBS, the fixation was stopped by incubating cells with 2 mg/ml glycine for 10 minutes at room temperature, followed by another three washes with PBS.
Cells were incubated on ice for 45 min in the dark and the reaction was stopped by washing cells twice in PBS/BSA 1%.
Additional experiments confirmed that the parasite was stopped by T cells, not by antibodies.
Stimulation was stopped by washing cells in ice cold PBS.
The reaction was stopped by washing cells twice with RPMI 1640, containing 10% FBS.
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