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Digestion was stopped by addition of fetal bovine serum.
Reactions were stopped by addition of reducing SDS sample buffer and boiling for 5 min.
Subsequently, activity assay was stopped by addition of 10 mM Na2CO3 and incubation on ice.
The reaction was stopped by addition of 900 μl of 150 mM sodium hydroxide.
After incubation the reaction was stopped by addition of 15 ml of 10% trichloroacetic acid (TCA).
The reaction mixture was stopped by addition of 5 ml of trichloroacetic acid (110 mM).
Reactions were stopped by addition of sulfuric acid, and absorbance was read at 450 nm.
Reaction was stopped by addition of 50 µl stop solution.
Development was stopped by addition of 1 M H2SO4.
The reaction was stopped by addition of 2× Laemmli buffer.
Reactions were stopped by addition of Laemmli buffer.
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