Sentence examples for stop solution with from inspiring English sources

OD values were read within 30 min of addition of the stop solution with a MRX Revelation microtiter plate reader (Thermo Labsystems, Franklin, MA) at a wavelength of 410 nm with a 610-nm reference filter.

Stop solution (supplied with kit) was added to all samples, positive control, and blanks.

As with the IL-12 kit, the reaction was terminated with stop solution, the absorbance measured on the VersaMax plate reader at 450 nm and the results calculated using the VersaMax curve fitting statistical software.

The reaction was terminated with stop solution and the optical density was measured photometrically at 450 nm.

The tissue supernatant fluids were added to each well, and treated with detection antibody, supplemented with substrate and stop solution.

The reaction was stopped after five minutes with the "stop solution" provided in the kit.

The reaction was terminated with stopping solution after 30min incubation and optical density (OD) was measured at 450 nm.

The reaction was terminated with stopping solution after 30 min incubation and optical density (OD) was measured at 450 nm.

The enzymatic digestion with 1 mg/ml (activity 0.199 U/mg) Collagenase B and 0.033 mg/ml Trypsin was performed in oxygenated Tyrode solution with 25 mM BDM and 50 µM CaCl2 for 8 12 min and after manually dissecting the tissue the reaction was stopped in stopping solution (oxygenated Tyrode solution with 25 mM BDM, 50 µM CaCl2 and 5 % FCS).

The assay was developed with BluePhos substrate (Kirkegaard Perry, Gaithersburg, MD) for 15 min at room temperature, then stopped with stopping solution (Kirkegaard Perry) and read at 570 nm (SpectraMax Plus384, Molecular Devices).

The reaction was stopped with stopping solution (Sigma, cat. # S5814), and the absorbance at 450 nm was measured using an ELISA reader (Bio-Rad).