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The reaction was terminated by stop solution.
Stop solution, 2 N H2SO4, was added to each well.
-Diluent 2: one vial contains lyophilized material of murine origin -Wash solution (20×): one 50 mL vial -Substrate: one vial -Stop solution: Stop solution is a tacrine solution.
The ELISA was stopped with 50 μl/well stop solution and read at OD 450 nm.
Stop solution (50 μl) was added, and the absorbance was measured at 492 nm.
The reaction was stopped by adding 50 μL of 2 N H2SO4 "Stop" solution.
A 1 4 dilution of "stop solution" to blood was vortexed and placed on dry ice.
After 30 min of incubation, stop solution of the kit (100 μl) was added.
2.5 μl of Stop Solution was added to stop the reaction.
Reactions were stopped by stop solution (KPL, Gaithersburg, MD).
The stop solution was discharged, and the cells were washed again with stop solution.
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