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When Six2-GFPCre mice are crossed with a floxed STOP reporter strain, Cre-dependent removal of the stop sequence in progeny leads to constitutive and heritable expression of a marker gene such that all mesenchyme-derived renal epithelial cells, from the Bowman's capsule to the junction of the connecting segment and collecting duct, are heritably labelled [ 18].
Tau GFPnLacZ, previously termed Tau mGFP (Hippenmeyer et al., 2005 ) and R26 tdTomato (Ai9, Madisen et al., 2010 ), floxed STOP reporter lines expressing GFP and nuclear βgalactosidase or tdTomato, respectively, in response to Cre recombinase action.
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Lysates prepared from cells expressing Flag-tagged GFHNS NSS) or GFHStop (Stop) reporters as well as cells lacking a plasmid (UT) were immunoprecipitated with anti-Flag antibodies.
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