Sentence examples for stop reactions from inspiring English sources

To stop reactions, tubes were shaken vigorously on ice and immediately centrifuged for 5 min at 5000g at 5 °C. 10 mL of supernatant was then filtered in a Whatman 40 filter and precipitated with 23.3 mL isopropyl alcohol, after which the supernatant was discarded and the pellet was dried in a vacuum drier and weighed.

The following day, the cells were treated either with ethanol (vehicle) or 10−7M dexamethasone (DEX) for 90 min. and cross-linked to stop reactions.

For the DNAse I experiments, DNAse I was added to a final concentration of 100 U/µl, samples were incubated for one hour at 4°C, and EDTA was added to a final concentration of 10 mM to stop reactions.

After 30 min, plates were washed with tap water to stop reactions and then air-dried.

After transcription mixtures had been incubated for 12 h at 37 °C, 5 μL of 100 mM EDTA was added to stop reactions.

167 mM EDTA was added to stop reactions before separating samples by thin-layer chromatography on Baker-flex Cellulose PEI sheets (J.T. Baker) for 45 minutes in 0.8 M LiCl, 1 M formic acid.

Thus, the timing of reaction interrogation and/or a reliable "stop" reaction are required for quantitative precision.

The mixture was incubated at 37°C for 15 min and heated to 85°C for 5 s to stop reaction.

Stop reaction by adding 30 ul of 2X Laemmli protein sample buffer.

Mixture was boiled during 1 h and placed in an ice bath for 10 min to stop reaction.

Trypsin ethylenediaminetetraacetic acid was replaced with soybean trypsin inhibitor (Sigma, Irvine, Scotland, United Kingdom) for 5 minutes at room temperature to stop reaction.