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The Bdnf stop mouse line was provided by Rudolf Jaenisch (Chang et al., 2006).
To generate the Ntf3 stop mouse, we engineered the conditional Ntf3 overexpression transgene using the mouse Ntf3 cDNA (kindly provided by Barbara Hempstead, Cornell University) under regulation by the synthetic CAGGS promoter/enhancer/intron followed by a loxP-STOP-loxP cassette (kindly provided by Laurie JaChildrenusby, Children's Hospital Boston).
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To overexpress Ntf3 from adult supporting cells, we crossed Slc1a3/CreER T mice with Ntf3 stop mice.
They further contest reliable impairments in non-associative learning in Mecp2 Stop mice.
To overexpress Ntf3 from hair cells, we crossed Pou4f3/CreER T :Rosa26 tdTomato mice with Ntf3 stop mice.
In contrast, light-period activity profiles during which the amount of sleep was highest remained normal in Mecp2 Stop mice.
Ambulatory activity (distance moved) was reliably lower in Mecp2 Stop mice (F 1,162) = 16; p = 0.001 for genotype, ns for time).
Overall, key values were lower in Mecp2 Stop mice underlining the notion of slower habituation and lower general activity.
To overexpress Bdnf or Ntf3 from postnatal supporting cells, we crossed Plp1/CreER T mice with Bdnf stop or Ntf3 stop mice, respectively.
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