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Somalia brought to a stop Krebs, a German trawler, by firing at her, forcing her to stop.
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Islets were trypsinized with 0.1 mg/ml trypsin (1∶250, Difco) and digestion was stopped with cold Krebs-Ringer-Bicarbonate-Hepes (KRBH) buffer-0.5% BSA (Interchim) and 5.5 mM glucose [13].
After a 30-min equilibration period in normal Krebs, preparations were incubated for 30 min in K+-free Krebs.
After incubation, the cells were rinsed three times with ice-cold Krebs-Ringer buffer to stop further cell uptake and then immediately lysed using 1 mL 5% trichloroacetic acid (TCA) for 1 h.
After incubation, cells were rinsed three times with ice-cold Krebs-Ringer buffer to stop further cell uptake and then immediately lysed using 1 ml of 5%% trichloroacetic acid (TCA) for 1 h.
"It is inevitable that the whole block will be changed," Mr. Krebs said.
The binding reaction was stopped by two rapid washes of 5 ml cold Krebs buffer.
Uptake was measured at variable time points up to 10 min and stopped by the addition of 2 ml ice-cold Krebs buffer (130 mM NaCl, 10 mM Na2HPO4, 4.2 mM KCl, 1.2 mM MgSO4, and 0.75 mM CaCl2, pH 7.4) and filtered through a 0.45 µM nitrocellulose filter under vacuum.
Father Krebs talked a bit about the people who stopped by while the bell tower was being erected.
Krebs called the police first.
How's your son Max?'" Krebs recalls.
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