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In the HAP1 hoc gene the transition C496→T occurs, thereby generating a nonsense mutation Gln166→ orche stop codon which was confirmed to stop incorporation of Hoc into the phage capsid [ 22].
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For BrdU staining, 2×106 MM cells were incubated with 10 μM BrdU in PBS for 30 minutes and afterwards ice-cold PBS was added to stop the incorporation.
In wild type embryos, about 5 hr AEL, the ventral epidermal cells incorporated BrdU in S15, whereas the lateral cells entered G215 and stopped BrdU incorporation.
Given these circumstances, many whites came to realize that there was no stopping the incorporation of blacks into the South African political system.
Cells that had left S phase could be noted by IdU+ only (IdU+CldU−) immunosignals, since they stopped nucleotide incorporation before CldU pulse labeling.
Like most neonate swordtails, Type I cichlids revealed a stop of calcium incorporation after unilateral vestibular nerve transection.
These integrations can result in the modification of transcriptional efficiency, as well as introduce transcript splice variation and polyadenylation sites, changes in transcription start and stop sites, and incorporation of novel exon sequence.
Genes encoding full-length GlLa or the C-terminal domain (211 348aa) of GlLa were each amplified from Giardia genomic DNA with a modification of the stop codon and an incorporation of a NheI site at the 3'-end.
The Hg Cu ratio (0.6 1.1 = 0.55:1) was higher than the theoretical limit set by the fact that the Hg incorporation should stop when all S S bonds are broken (0.33, see the Cd case discussed earlier).
Amino acid incorporation was stopped by the addition of 60 μl of cicloheximide (20 mg/ml) and ice incubation.
CFSE incorporation was stopped by adding complete medium and the cells were washed once with complete medium.
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