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Aliquot of the solution was then transferred to PBS with a final concentration of 2 mM EDTA to stop further degradation and DNA content was quantified using picoGreen (Invitrogen, Grand Island, NY,) according to the manufacturer's instructions.
To stop further degradation due to protease digestion a solution of 100 mM phenylmethanesulfonyl fluoride (PMSF), 100 mM N-ethylmaleimide (NEM) in propan-2-ol to a final concentration of 10 mM was added.
Aliquots of the solution containing NETs were then transferred to PBS with a final concentration of 2 mM EDTA to stop further degradation and DNA content was quantified using PicoGreen (Invitrogen).
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The problem can be partially resolved by the formation of Cr2O3 protective coating onto the metal scaffolding which effectively stops further degradation and retains good electrical conductivity.
But it can stop further injury.
This is likely to slow or even stop further reforms.
There is an endeavor to stop further CS rise.
He requested for medicines that would stop further retraction.
The objectives of this transformation process is the reduction of heavy environmental pollution, primarily the reduction of SO2- and airborne particles and past pollutions, as well as the mine reclamation stopping the further degradation of settlements with technical infrastructure.
The setback has not stopped further prosecutions, however.
Point of saturation was the criterion for stopping further discussions.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com