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After self-healing of the damage, the microvascular membrane's rejection was restored back to 82% of its original value without a need to stop filtration.
The uptakes of [14C]-butyrate were measured by rapid stop filtration technique.
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However, ligands with bromide counter ions were dissolved in methanol following the same procedure, stopping at till filtration.
Incubation was stopped by rapid filtration and rinsing with ice-cold binding buffer, using the Brandel harvester (Gaithersburg, MD) and Whatman GFB filter paper (Brandel, Gaithersburg MD).
The reaction was stopped by rapid filtration and the plates were washed twice using 200 μl of buffer.
The reaction was stopped by vacuum filtration through Whatman GF/C glass fiber filters (Whatman, Maidstone, England) using a Brandel M24-R Cell Harvester (Brandel, Gaithersburg, MD, USA).
In hypovolemic states, the fall in Piv leads to a transient back-filtration of fluid from the interstitial space in the intravascular space; as a result, Piv progressively increases until filtration stops.
The reaction was stopped by gel filtration over a PD-10 column (Amersham Biosciences, Uppsala, Sweden) and the biotinylated protein concentration was determined by spectrophotometry at 280 nm (ε280=1.4 mg−1 ml−1).
Check the filtration system daily.
In classical probability theory, a random time T is a stopping time in a filtration (Ft t⩾0 if and only if the optional sampling holds at T for all bounded martingales.
Samples were incubated for 90 min. Incubation was stopped by rapid vacuum filtration over Whatman GF/C glass microfibre filters (VWR International SAS, Strasbourg, France) presoaked with polyethylenimine 0.3% in order to reduce non-specific binding to the filters.
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