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Five new alleles of Cyclin E were recovered, ranging from a missense mutation that is viable to stop codons causing embryonic lethality.
Mutation of the resume codon that allows the switch of template of the ribosome required for its release was essential in vivo, however a mutant in which this codon was followed by stop codons interrupting the tag sequence was viable.
h22 is a lethal EMS-induced null allele encoding a truncated protein of only 42 amino acids due to a premature stop codon; h674 is a lethal EMS-induced hypomorph allele encoding a protein lacking the C-terminal 103 amino acids due to a premature stop codon and h1J3 and h1 are viable alleles with inserted transposable elements ([26], [27]; Flybase).
Most invasions, if detected early, can be stopped, because establishing an organism so it's viable in a new environment is not automatic.
Lmna−/− mice are viable, but exhibit growth retardation from 2 3 weeks of age, and stop growing after ∼4 weeks.
Neither outlook is viable.
"Will it be viable?
Now both are viable.
But many were viable.
All were viable options.
"It may not be viable.
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