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Stool specimens (280) from CCF pediatric patients were evaluated for both rotavirus and BEP.
Results: Among 200 stool specimens, 197 were in accord by PTH, RTC and CCA-Ag.
Results: Overall, REIAs were performed on 81% (538/661) of stool specimens, with 37% positive.
Stool cultures were performed on 62% (408/661) of stool specimens, with 4.4% positive.
Stool specimens were stored at −20°C until used.
We were unable to obtain the HIV status of patients from whom stool specimens were submitted.
Our study reports a high proportion of stool specimens with no identifiable pathogenic bacteria or parasites.
Biopsy specimens were also disrupted and homogenized following the same procedures as the stool specimens.
DNA was extracted from stool specimens using the Stool kit according to manufacturer's protocol.
Resuspended stool specimens were strained through cheesecloth.
Otherwise, fresh stool specimens were used.
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