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Stocks of cells that were resistant to hygromycin were prepared and frozen.
Permanent stocks of cells were prepared and all cells were stored at −80 °C until use.
When purchased, permanent stocks of cells were prepared and all cells were stored at −80 degrees until use.
The cells were used at the lowest passage numbers that would provide adequate stocks of cells, they were checked as mycoplasm free, and they were authenticated from source, as required by the UKCCCR ' Guidelines on the use of cell lines in cancer research' (< http://ukcccr.icnet.uk>).uk>
Cultured cells were grown at 39°C in a 5% CO2 incubator until cells reached confluent monolayers (2 to 4 days) and primary CEF cells were passaged every 3-4 dand and frozen stocks of cells were prepared from each passage at a density of 3 × 106 cells and stored in liquid nitrogen.
Similar(55)
Stocks of cell lines were frozen and kept at − 80 °C until required.
Infected cell culture media were cleared of cell debris by low-speed centrifugation and collected as stocks of cell-free virus.
The cell lines were cultured bi-weekly and stocks of cell lines were passaged no more than ten times for use in experiments.
Prior to manuscript submission, genomic DNA from frozen stocks of cell lines were submitted for short tandem repeat analysis [ 24] at RADIL University off Missouri).
6- 11 Use of the hepatoma line Huh-7 and its derivatives and adaptation of viral genomes to propagation in these cells has made possible the generation of high titer stocks of cell culture-derived HCV (HCVcc), 12, 13 enabling the identification of cellular factors required for virus entry and replication.
Stock cells lines were maintained in the absence of penicillin and streptomycin and screened for mycoplasma prior to preparing a stock of cells that were frozen for use in experiments.
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populations of cells
components of cells
sections of cells
catches of cells
sectors of cells
positions of cells
actions of cells
capabilities of cells
steps of cells
inventory of cells
action of cells
share of cells
stocks of home-builders
stocks of narcotics
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stocks of spares
stocks of arms
stocks of goods
stocks of retailers
stocks of companies
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