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Serum and whole blood was stocked frozen until analyzed.
Plasma and whole blood was stocked frozen until analyzed.
One xenograft was subsequently transplanted from mouse to mouse and stocked frozen in DMSO-fetal calf serum solution or frozen dried in nitrogen for further studies, and a fragment was fixed in phosphate-buffered saline (PBS) 10% formol for histological studies.
Stocked frozen clinical isolates had a slightly higher frequency of pVir than fresh clinical isolates (18% vs. 13%, respectively), most likely because of sampling error.
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Soon, mainstream stores began stocking frozen Indian entrees like kafta curry, palak paneer and samosas, many bearing the Deep Foods label.
You can buy fresh gnocchi in specialty-food stores, and it has become popular enough so that some conventional groceries also stock frozen and dried versions.
I go to lots of Greek, Arab and Turkish grocers, most of which stock frozen packs of the stuff, and always end up coming away with all sorts of other ingredients that I can't get in normal supermarkets, notably fruit and veg that tastes as it's meant to and all sorts of young cheeses.
A judge has ordered all of the assets derived from the sale of Wellness stock frozen, but only the elder Pappas and his two companies have been charged with fraud.
The microorganisms and rice leaves and seeds used are listed in Tables 1 and 2. Yeast stock cultures were obtained from stock frozen at -80°C and were incubated at 30°C for 3 days on malt-yeast-glucose-peptone (YM) agar medium containing 1% glucose, 0.5% peptone, 0.3% yeast extract, 0.3% malt extract and 1.5% agar.
Injection inocula were prepared from glycerol stocks (frozen at OD600 = 0.75) by washing three times in sterile 0.05% Tween80/PBS solution (BD Difco), assessing optical density at 600 nm and resuspending in a 2% polyvinylpyrrolidone40 (PVP40) solution (CalBiochem) in PBS.
LNCaP cells from the master stock (frozen in Aim V media (Invitrogen) plus BSE-free fetal bovine serum (20% FBS; HyClone) and 5% DMSO (Edward Life Sciences)) were expanded in Aim V media/1% FBS, and cells were treated with UV-B irradiation such that >90% of cells underwent apoptotic death (Caspatag positive, as described[29]).
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