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These standards were used to prepare stock standards solutions.
Standard solutions were prepared from 500 μM stock standards of each analyte and were made up in 0.1 M perchloric acid.
The concentrations of analytes such as nitrate and nitrite were determined referring to calibration curves constructed with water solutions prepared by opportune dilution of stock standards (Certipur from Merck, Milan, Italy) containing 1000 mg/L of each analyte.
The calibration curves of the standards were made by a dilution of the stock standards (five set of standard dilutions) with methanol to yield 1.0 5.0 μg/ml for GA, CH, VA, EC, PCA, EA, MC, KF 0.5 4.0 μg/ml for CH, CA, RH, and 0.25 3.0 μg/ml for QU.
Besides, stock standards solutions of sulphonamides, penicillins, macrolides, and tetracyclines were prepared by dissolving 10 mg of each substance into 10 mL of methanol, except for penicillins which were dissolved in 10 mL of Milli-Q water (Millipore corp., Bedford, MA, USA).
Stock standards were: Aggrecan from bovine articular cartilage (cat. no. A1960; Sigma-Aldrich) digested with ADAMTS-4.
Similar(44)
This, despite the fact that 90% of the letters he receives are "stock standard".
Label as Stock standard A. (ii).
Discard the other stock standard solution.
Stock standard solutions were prepared from Analar R grade salts.
Calculate exact concentrations of each component in stock standard solution as shown below: Stock standard A use concentration as is.
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