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AuNP working concentrations were prepared from the 1,000 μg/ml stock preparations in EMEM/S + or EMEM/S-.
All stock preparations were stored at 4°C.
Aliquots of the liposomal stock preparations (diluted to 10 µM) were incubated with various concentrations of the rBPI21 to be tested at 25°C, and fluorescence was recorded continuously for 30 min, under stirring (wavelength of excitation, λexc = 492 nm; wavelength of emission, λem = 517 nm).
Enzyme solutions for the titrations were taken from previously purified enzyme stock preparations.
We thank Mr. Mike Smith for technical assistance in PCR, bacterial stock preparations.
CV-1 cells were utilized for virus titer determinations and TK-143B cells were used in virus stock preparations.
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Virus stock preparation was prepared by amplification of R5-HIV-1BaL and X4- HIV-1NDK on MDM and PHA+ IL-2 activated PBMC from healthy donors, respectively.
Stock preparation: tetracycline stock was prepared by dissolving tetracycline HCl in sterile distilled water at the stock strength 1 mg/mL.
Papermaking starts from wood handling and stock preparation.
The principal steps of papermaking include stock preparation, forming, pressing, drying, calendaring, and reeling respectively.
Several solution systems were used for NP stock preparation and analyzed during this experiment.
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