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The X. hellerii (Sarabia) [pedigree 11317] stock is maintained by reciprocal cross breeding between two distinct X. hellerii strains differing by sword color (orange or green sword).
Since Rp1-D21 homozygotes in the H95 background are often unable to set seed, this stock is maintained in heterozygous condition by repeatedly crossing it as a male to the H95 inbred.
HR may present benefits over total hip replacement because femoral bone stock is maintained, there is reduced wear compared with high density polyethylene, it has a large femoral head that could be reduced dislocation rate, it is said to offer the patient increased levels of postoperative activity and is easy to convert into a stemmed prosthesis [ 2].
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Heterozygous prg-1 / unc-13 males stock were maintained by backcrossing heterozygous males with unc-13 homozygotes.
Heterozygous nrde-1 / dpy-17 males stock were maintained by backcrossing heterozygous males with dpy-17 homozygotes.
Each stock was maintained at constant parental density for at least two generations to minimize environmental effects.
The *AB wild-type strain was used for these experiments and the merovingian (mero w40) mutant stock was maintained as heterozygotes in the *AB wild-type background.
From this starting population, an outbred stock was maintained by mating random pairs of beetles, with each individual engaging in only 1 breeding attempt per generation.
The stock was maintained in large cages at high population size (typically more than 200 individuals per cage) and with a 1 1 sex ratio.
Fly stocks were maintained in 250 ml bottles in uncrowded conditions.
Fly stocks were maintained at 25°C on standard cornmeal-based medium, supplemented with yeast.
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