Sentence examples for stock cells from inspiring English sources

The stock cells were maintained in 75 cm2 tissue culture flask.

Stock cells of Yarrowia spp. (Idd1 and Idd2), previously isolated and deposited to Japan Collection of Microorganisms under Accession no.

For the preculture, the stock cells were cultivated for 24 h at 30 °C with shaking in 4 mL medium (pH 6.2) containing 50.0 g L−1 d-glucose, 10.0 g L−1 peptone (Nihon pharmaceutical, Tokyo, Japan), 5.0 g L−1 yeast extract (Bacto Yeast Extract, Difco Laboratories, Franklin Lakes, NJ, USA), 4.0 g L−1 KH2.04, 2.0 g L−1 K2HPO4, 0.6 g L−1 MgSO4·7H2O.

Colonies resistant to puromycin appeared within 2 weeks, and the cells were then expanded for another 3 weeks to make the original stock cells.

Stock cells were grown as adherent monolayers in 75 cellculturelture flasks (Corning/Costar, High Wycombe, UK) and maintained at 37°C in a humidified atmosphere of 5% CO2 in air.

Stock cells were preserved in liquid nitrogen.

The cell suspension was further diluted as required using standard media to prepare a stock cell suspension of 50,000 cells/mL.

Stock cell cultures were grown in medium containing RPMI 1640, supplemented with 5% of fetal bovine serum.

All stock cell lines were grown in 90 mm dishes in DMEM supplemented with 10% calf serum (D10CS) (Gibco/BRL Co. USA).

Glycerol stocked cells were inoculated into mM63 medium [12] and grown until OD600 = 0.5 with shaking at 130 rpm.

To prepare virus stocks, cells permissive for the virus to be amplified (Vero-Slam, Vero, HepG2 (ATCC HB-8065), and Madin-Darby canine kidney (MDCK)) were infected and incubated at 37°C.