Sentence examples for stock cell from inspiring English sources

This could include using more organic composts and fertilisers, slowing the flow of water, fencing off waterways from stock, cell grazing, feral animal control and direct-drilling crops into pasture.

The cell suspension was further diluted as required using standard media to prepare a stock cell suspension of 50,000 cells/mL.

Bacteria were grown by plating one loop (1 µl) of stock cell suspension (heavy suspension of Burkholderia spp. in defibrinated rabbit blood, stored at −70°C until use) on trypticase soy agar with 5% defibrinated sheep blood (SBA) (BBL Microbiology systems, Cockeysville, MD) and incubating aerobically 1 2 days at 37°C.

Stock cell cultures were grown in medium containing RPMI 1640, supplemented with 5% of fetal bovine serum.

All stock cell lines were grown in 90 mm dishes in DMEM supplemented with 10% calf serum (D10CS) (Gibco/BRL Co. USA).

Furthermore, the FLK stock cell line has a signature base substitution (bp 5194; GenBank accession no. EF600696) in the env region; this substitution is unique among sequences deposited in GenBank and was not detected in the human BLV isolates we investigated.

Stock cells were grown in T150 flasks (Corning) in F12K media (HyClone) supplemented with 5% FBS (Sigma).

First, we question the trial's use of allogeneic induced pluripotent stem (iPS) cells, called iPS stock cells and prepared by Kyoto University's Center for iPS Cell Research and Application.

Cells were stained with PI for at least 30 min and run on the Imagestream X. Cell culture conditions and growth media were as previously described by Moreno et al.24 PN1 (wild-type haploid, strain 972h− mating type, lab stock) cells were grown in YE4S media and maintained in exponential phase.

All cells were cultured as described27,28, were maintained at 37 °C in a humidified atmosphere of 5% carbon dioxide with biweekly media changes, were drawn from stored stock cells, and replenished on a standardized periodic basis and were routinely monitored for Mycoplasma (PlasmoTest™, InvivoGen, San Diego, CA), at least every 3 months.

The stock cells were maintained in 75 cm2 tissue culture flask.