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EICs often contain significant amounts of variation, particularly for lower intensity species due to the stochastic limitations, microinstabilities in the electrospray process, and other sources of measurement noise.
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Minor topological and distance inconsistencies may occur across segments in phylogenies even without a reassortment event, due to stochastic errors and limitations in distance estimation methods.
We showed that various limitations of stochastic approaches, such as interference statistics at eccentric user locations or a certain degree of regularity among the node locations, can be resolved by deterministic structures.
Computational methods and, in particular, stochastic simulations overcome these limitations and provide a more accurate mechanistic representation of the underling biological process.
While these estimates are the only ones published to date on the efficacy of this strategy during an influenza pandemic, the inherent limitations of stochastic models such as the one used by Longini et al. must be acknowledged when this strategy is considered in practice.
One limitation of stochastic optimal power flow has been that only steady-state variable limits have been used as security constraints.
Although deterministic relaxation is relatively less computationally intensive compared to stochastic relaxation, a main limitation is that the relaxation process may get trapped in local optimal states more easily.
This is important, as slow runtime is often a limitation of stochastic methods.
This is not really a limitation because stochastic random walk motion yields the same diffusion behavior as a continuous particle density.
Another common limitation of stochastic procedures is that the computational cost is often by orders of magnitude higher than the deterministic analysis.
Computational power is a major limitation of stochastic methods, particularly when large data sets are involved, however this is dissipating rapidly with the improvement in processor speed, parallelization, and the adoption of 64-bit technology, however in the meantime deterministic methods will continue to be used for large scale BLUP analysis.
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