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The solvent was evaporated by heating the mixture on a magnetic stir plate and stirring with a Teflon-coated magnet.
Next, 1.2 mL 5%% (wt) sodium citrate was added under rapid stirring with a magnetic stir bar.
Cells were constantly mixed by stirring with a magnetic stir bar (7 cm length) at 250 rpm.
4. Add the butter and cook, stirring with a wooden spoon, for 1 to 2 minutes, then stir in the cheese.
Stir 1/4 cup of all-purpose flour into the onion mixture and cook, stirring with a wooden spoon for 1 minute.
Bring to the boil, stirring with a wooden spoon.
Spoon in grits, stirring with a wooden spoon.
Add the flour and cook for two minutes, stirring with a whisk, to make a roux.
Simmer, stirring with a wooden spoon, until thick enough to coat back of spoon.
Cook for about 3 minutes, stirring with a wooden spoon to prevent the mix from catching.
Add flour, and cook until a dark golden brown, 2 minutes, stirring with a wooden spoon.
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