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Place the mixture over a medium heat while slowly stirring using the wooden spoon.
The stable aqueous suspension was then exposed to the UV-radiation with continuous magnetic stirring, using the home made photoreactor containing two 18-W tubes as the UV-source (λ = 200 to 400 nm).
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Then, the crosslinker solution and the graft nanocomposite dispersion were mixed and homogenized by constant stirring using a magnetic stirrer to obtain the gelant solution.
The receptor fluid was kept at 37 ± 0.5°C with constant stirring using a Teflon-coated magnetic stir bead.
After stirring using a VWR Analog Vortex mixer for 35 min, the extract was decanted into another vial.
Stirring: Using a long metal or glass rod spoon, ingredients are stirred together, mainly using an old fashioned or highball glass.
The resulting solution was heated to 80°C under stirring using a mechanical stirrer.
A four percent dispersion of NaCMC was prepared by swelling the polymer in distilled water for 24 h and subsequently stirring using Ultra-turrax (T 25 basic, IKA, WERKE, GmbH&Co.KG, D) for 2 min (16,000 rpm).
4. Raise heat to medium-high, add sausage and cook, stirring, using a wooden spoon to break up clumps.
The desired amount of aqueous solution of cobalt nitrate and iron nitrate were taken and subjected to stirring using a magnetic stirrer for half an hour.
Eluted fusion protein was concentrated to approximately 2 mg/mL under nitrogen pressure (40 50 psi) with stirring, using an Amicon Ultrafiltration membrane (30,000 MWCO; Millipore, Billerica, MA).
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