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SS solution (20 wt.%) and SF solution (10 wt.%) were prepared by stirring the samples in trifluoroacetic acid (TFA) at 25°C for 3 h.
Moreover, a practical flow-process system can be easily used with the low-viscosity OES to pretreat cellulose, which could save energy in pipeline transport and stirring the samples.
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After stirring, the sample was filtered through 0.45 μm pore size filter.
A solution of 0.01 g of P DLLA-co-CL) in 5 mL of chloroform has been obtained by stirring the samP DLLA-co-CL at room temP DLLA-co-CLa closed vessel.
Enzymatic treatment was carried out by stirring the sample (same conditions as before) in 15 ml of washing medium with 0; 0.15 or 0.37 mg/ml collagenase (Sigma) for 30, 60 or 120 min at room temperature.
After vigorous stirring, the sample froze instantly.
After gentle stirring, the sample was allowed to settle for a few hours to overnight, permitting the organic and aqueous layer to separate.
Vigorous stirring ensured the sample's homogeneity and that the X-ray beam (1 mm diameter) passed through a representative sample of the ampoule's contents.
just stirring the pot.
Continue stirring the mixture.
Begin stirring the mixture.
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