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I realized there was a stirring down there, so I encouraged that stirring.
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The statement was greeted with thunderous applause, stirring Downing to issue an ill-considered statement of her own calling Chandler "angry and bitter" and lamenting that it was "too bad when some people get old, they get bitter".
Sparging was totally interrupted 48 h after inoculation, and stirring slowed down to 200 rpm.
The pellet was resuspended in 5 mM MgSO4 and the cells were placed on ice for 10 min with slowly stirring, pelleted down at 8000 × g for 15 min at 4°C and the supernatant collected for purification.
Thankfully, President Bushs stirring throw-down-the-gauntlet speech in August at the Veterans of Foreign Wars convention and his surprise visit to Iraq in September demonstrate that the Commander in Chief has no intention of undercutting our newfound successes in Iraq.
Thankfully, President Bush's stirring throw-down-the-gauntlet speech in August at the Veterans of Foreign Wars convention and his surprise visit to Iraq in September demonstrate that the Commander in Chief has no intention of undercutting our newfound successes in Iraq.
As you look more closely, this figure appears, face down, stirring like mounds from the sand, like somebody covered in sand, or made loosely from sand.
Now, using a batter very similar to the muffin dough, but much more liquid – note: the first is a dough, the second is a batter – and adding, at the stirring-down stage, a little extra warm water in which a small quantity of bicarbonate of soda has been dissolved, the mixture works out just about right.
If the mayo seems very stiff, "let it down" by stirring in a little warm water.
If this happens, "let it down" by stirring in a tablespoon or two of warm water (a handy trick for any over-thickened mayonnaise).
At day 7, cells were mixed with a P200 multichannel pipette set at 40 μl (pipetting 10 times up and down while stirring in a circular motion to ensure cells on the edges of the well bottoms were mixed); then, using a P20 multichannel pipette, 10 μl was spotted onto the surface of the 7H11-OADC-charcoal agar plates (CARA microplates).
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CEO of Professional Science Editing for Scientists @ prosciediting.com