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In this study, a polysulfide rubber emulsion was synthesized through the pre-emulsification of polysulfide rubber by mixed-emulsifiers and polyvinyl alcohol as a weight stabilizer and the emulsification by adding deionized water dropwise at a speed of 1 ml/min, stirring at a speed of 1500 rpm and adjusting pH to 8 by ammonia.
The drilling fluid property was tested after 20 min of stirring at a high rate.
The solution of each experiment was placed in a (Pyrex) glass reactor and set on a heating plate with automatic temperature control and mechanical stirring at a rate of 750 min−1 to avoid particle fragmentation.
An amount of 0.39 g (2.3 × 10−3 mol) of AgNO3 was added, and the mixture was heated while stirring at a temperature of 70 75 °C, then a 100 mL aqueous solution of 1.88 g (6.96 × 10−3 mol) of K2S2O8 was added dropwise to the mixture.
After that, the bags were suspended in a beaker containing 7.5 mL of PBS under stirring at a speed of 500 rpm.
In the case of the ATHF of N-tosyl 3-pyrroline, we observed a minor side-product arising from isomerisation and subsequent hydroformylation.[ 4d, 6] Seeking to eliminate this, we investigated starting the reactions at high temperature to generate syngas, then stirring at a significantly lower temperature while hydroformylation took place.
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The first method was stepwise addition of ~1.0 mg (at a concentration of >10 mg/ml) of denatured protein into 100 ml of Buffer E while stirring at 4 °C; a total of 4 or 5 20-μl additions were made.
As Lemann shows, this is an idea that has been stirring at ETS for a while, generating strong internal opposition.
A solution of (NH4 2HPO4 (300 mL, 10 mM) was added dropwise into a solution of Ca(NO3 2 (500 mL, 10 mM) under rapid stirring at 4 °C in an ice water bath for a final calcium to phosphate ratio of 1.67.
The protein solutions (25 ml) were adjusted to pH 8.0 with 0.1 N HCl or NaOH, while stirring at 37°C in a water bath.
After 24 h of stirring at room temperature, a float-sink separation was performed by centrifugation (3500 rpm for 20 min).
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