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To a 50 mL rounded-bottomed flask equipped with a magnetic stirrer was added copper iodide (0.19 g, 1 mmol), DMEDA (1.728 mL, 8 mmol), potassium carbonate (4.15 g, 30 mmol) in the nitrogen atmosphere.
To maintain platelets in suspension, a magnetic stirrer was added to each sample (stirring rate 1 200 rpm).
Following the desired length of time, the incubated protein sample was removed and 1 mL of phosphate buffered saline (PBS, 10 mM pH 7.3) and a magnetic stirrer was added followed by 2 h of gentle stirring to ensure dissolving the soluble fraction of the sample.
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When Iron solution was agitated by stirrer, it was added drop by drop to it 100 ml urea 1 M (as a supplying agent of hydroxyl ions).
Briefly, after the appropriate treatment, 1 mL of cell suspension was gently stirred with a magnetic stirrer before FDA was added to a final concentration of 12 µM.
In the experiments where the effect of diluting the initial polyelectrolyte/surfactant mixtures (with or without 400 ppm of the silicone emulsion) was studied, the appropriate mixed solution was equilibrated for at least one day under agitation with a magnetic stirrer before it was added to the sample cell.
After sonication, the mixture was stirred continuously via magnetic stirrer and APS solution was added drop wise as an oxidizing agent.
To 100 mL ultrapure water in a glass beaker containing a small stirrer bar, 0.02% (v/v) acetic acid was added and mixed well on a magnetic stirrer at 60°C in a fume hood; ∼2% (v/v) 3-mercaptopropyltrimethoxysilane (TSL8380, Toshiba GE silicone, Toshiba, Tokyo, Japan) was then added with a glass pipette and mixed.
After vigorously stirring for 5 min (the rate of stirring was such that the solution vortex reached the base of the stirrer), 20 mg of NaBH4 powder was added rapidly to the precursor solution.
Then, a solution of 0.5 mmol of phenanthroline in 2-propanol was added over a stirrer.
First, 0.5 mmol of lawsone was dissolved in 20 mL of 2-propanol, and 0.5 mmol of triethylamine was added over a stirrer.
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