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The stabilization by wet stirred media milling was realized in a Minicer laboratory stirring media mill (Netzsch, Germany).
This enables simultaneous precipitation and stirred media milling.
The chapter shows possibilities to develop new and optimize existing grinding processes with stirred media mills.
As stirred media mills are operated usually wet, the chapter focuses on wet grinding.
Tissue culture inserts are held suspended in stirred media (top left).
b The basic MCL growth setup showing the insert suspended in stirred media.
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The mill with stirred ball media was chosen for the ultra-fine liberation of the coal components.
Growth in stirred liquid media occurs as an even cell suspension, making the cultures easy to divide and transfer, although isolating single bacteria from liquid media is difficult.
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