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Chitosan-based silver nanoparticles was prepared by dissolving 10 ml of 52.0-mM AgNO3 solution in 10 mg ml−1 of the prepared chitosan solution (2 % v/v in acetic acid), then stirred until homogenous.
All ingredients of the ointment base were mixed and heated gently, with stirring until homogenous and then stirred until cooled.
The mixtures were stirred vigorously by magnetic stirrer at 60 °C for 12 h until homogenous composites solutions were observed (see Fig. 1 ii) for the case of rGO/EPDM).
Slowly add the remaining liquid, mixing until homogenous.
Stir until just dampened.
Stir until moistened.
Stir until butter melts.
Stir until melted.
Stir until well incorporated.
Stir until eggs set.
Stir until yeast dissolves.
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