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The small number of dentists recruited could minimise the bias resulting from any unfamiliarity on the part of the participating dentists with the stipulated treatment protocol.
At the end of the stipulated treatment period, the overnight fasted (water allowed) animals were anaesthetized, blood samples were collected by retro-orbital puncture in heparinised (for haematological and biochemical analysis) and non-heparinised tubes (for serum electrolytes).
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Despite these relatively high frequencies of severe toxicity per patient, adherence to the protocol-stipulated treatment delays and dose reductions resulted in low rates of severe toxicity per chemotherapy cycle.
Electronic capture of both investigator-prescribed and patient-administered insulin doses, along with tracking records of the algorithm-recommended dosing, provided a unique opportunity to evaluate adherence and to better understand deviations from protocol-stipulated treatments.
Cells were washed once in ice-cold PBS and lysed in whole-cell lysis buffer (20 mM HEPES pH 7.5, 350 mM NaCl, 0.5 mM EDTA, 1 mM MgCl2, 0.1 mM EGTA, and 1% NP-40) after stipulated time of treatments and boiled at 95 oC with Laemmli's SDS-PAGE sample buffer for 5 min.
Cells were washed once in ice-cold PBS and lysed in whole cell lysis buffer (20 mM HEPES pH 7.5, 350 mM NaCl, 0.5 mM EDTA, 1 mM MgCl2, 0.1 mM EGTA and 1% NP-40) after stipulated time of treatments and boiled at 95°C with Laemmli's SDS-PAGE sample buffer for 5 min.
A treatment plan, which stipulated proton beam therapy and chemotherapy, was agreed for Ashya by the court, which left the NHS no alternative but to agree to pay under the S2 reciprocal funding arrangements between European Union member states.
The cells were lysed after stipulated time of growth factor treatment using the lysis buffer for containing 6M urea.
This treatment duration was stipulated in the reimbursement policy of the National Health Insurance in Taiwan at that time, but treatment duration is now determined by response to therapy.
The majority of relatives stipulated that adherence to legislation, treatment benefit for the study patient and for future patients, no patient-risk or -discomfort and development of new drugs were important factors if their relative was to participate in an ICU drug trial.
A consent form for a recent study of Genentech's immunosuppressant drug Raptiva told participants that they would be treated for any injuries the drug caused, but stipulated that "the cost of such treatment will not be reimbursed".
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