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A-tDCS stimulation was generated by a specially designed MRI-compatible neuroConn stimulator system (Rogue Resolutions; http://www.rogue-resolutions.com) and delivered at 2 mA continuously for 20 min via a pair of identical MRI-compatible leads and rectangular rubber MRI-compatible electrodes (5 × 7 cm), allowing for a current density of 0.057 mA/cm.
The LED stimulator system was developed as an open-source project.
This implantable stimulator system did not hinder animal movement or hygiene, and enabled the animals to be housed in regular animal facilities, since neither external equipment nor an externally generated magnetic field was required.
We applied oscillating currents at the IAF of each participant using a battery-operated stimulator system (Eldith, Neuroconn, Ilmenau, Germany).
Each rat was placed under an inverted clear plastic chamber on the glass surface of the Paw Thermal Stimulator System (UCSD, San Diego) and allowed to acclimatize for 30 min before the test.
Tumour-bearing mice were acclimated on the glass surface of the chamber of the Paw Thermal Stimulator System (UCSD, San Diego, CA, USA) for 60 min before the test (Hargreaves et al, 1988).
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The TMS stimulator consisted of a Focal Bipulse figure-of-eight coil (mean/outer winding diameter ∼50/70 mm, biphasic pulse shape, pulse length ∼280 µs, focal area of the stimulation 0.68 cm) driven by a Mobile Stimulator Unit (eXimia TMS Stimulator, Nexstim Ltd .. We controlled TMS parameters by means of a Navigated Brain Stimulation system (Nexstim Ltd).
The Compex Motion stimulator represents a further evolution and expansion of the ETHZ-ParaCare functional electrical stimulation system.
Full-field ERG was recorded by Burian Allen corneal contact lens electrodes with an EOG-ERG Ganzfeld stimulator (Electrophysiology system; LACE Elettronica, Pisa, Italy) according to the recommendation of the International Society for Clinical Electrophysiology of Vision (ISCEV) [ 16].
At the start of each run, the two probe tips were driven towards the skin sites until each tip registered a force of 0.1 g, as determined by a closed-loop algorithm in the CM-1 stimulator feedback system.
A 2100 isolated pulse stimulator (AM-systems, Carlsborg, WA) gating a GRASS S44 Solid State Wave Generator stimulator (GRASS Instruments, Quincy, MA) connected to a caliper electrode (BTX, San Diego, CA) was used to perform the EP.
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