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This necessitated using T cell receptor and CD28 antibodies stimulation to detect substantial amounts of HIV-1.
More frequent stimulation to detect earlier onset was not possible without active skin cooling.
LTP was induced by either one or four trains of tetanic stimulation (100 pulses/100 Hz; intertrain interval, 2 s) and monitored for 4 5 h after the tetanic stimulation to detect late-phase LTP.
Cells (5 × 10 cells/well) were spread onto 96-well plates and incubated for different days in cell culture media in the presence of serum combined with 100-ng/ml EGF stimulation to detect.
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We performed a low-rate repetitive nerve stimulation protocol to detect transitory depression of the compound action potential, which represents the neurophysiologic counterpart of transitory weakness (Aminoff et al., 1977).
This effect does not appear to reflect a better differentiation between stimulation and no stimulation, but suggests an increased readiness to detect stimulation, even when there was none.
In a study comparing the sensitivity of paresthesia and a motor response to nerve stimulation with an electrical charge of 50 nC or less, the sensitivity to nerve stimulation was 74.5% to detect needle-nerve contact as observed by ultrasound [ 6].
An aliquot of supernatant was taken after 24 h of stimulation in order to detect IL-2 production.
We also included an expression analysis 24 h after IFNα stimulation in order to detect microRNA genes that show either delayed induction or remain activated at comparable levels to the 4 h stimulus.
This assay is suitable for use with splenocytes from some mouse strains (129/SvEv), but not others (C57BL/6J), probably due to C57BL6J producing insufficient amounts of IFN following stimulation to be detected by intracellular staining.
First, we necessarily had to stimulate deep brain targets with a monopolar electrode configuration so as to detect stimulation timing from the stimulus artefact recorded from the scalp.
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