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It was necessary to exclude the possibility that the residual NK-cell population in IL-15−/− mice had acquired IL-15-independent, alternative modes of activation and therefore did not exhibit the same stimulation requirements as NK cells in WT mice.
We re-examined stimulation requirements for neuropeptide action using the selective stimulation and intrinsic detection in the OHN→HAN circuit across OHN activity linked to behavior in vivo (1 20 Hz firing, Adamantidis et al., 2007, Lee et al., 2005, Mileykovskiy et al., 2005).
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Previous studies have suggested different underlying mechanisms for this continuous stimulation requirement.
Changes in surface marker expression reflect changes in T-cell function, circulation pathways, and co-stimulation requirements, for example.
Monoclonal antibodies, 10 µg/ml, were added where indicated to test for MHC restrictions or co-stimulation requirements of the anti-ergotypic T cells.
T-cell activation or differentiation state has been shown to differentially affect co-stimulation requirements.
There is some controversy in the extant literature regarding the co-stimulation requirements of 'memory' CD8 T cells (Flynn and Mullbacher, 1996; Borowski et al., 2007).
A significant contributor to high stimulation threshold requirements is poor biocompatibility.
In the case of strategically patterned stimulation the requirements are even more stringent, for the brain signal must not merely correlate with clinical state but must also cause it in order to be a legitimate target.
To our knowledge, the results presented herein are the first to link the stimulation and requirement of ERK1/2 with nanoparticle stimulated autophagy.
Our studies indicate that in young skeletal muscle, SOCE contributes to maintenance of contractile force under high frequency stimulation, where Ca2+ requirements may exceed the capacity of the intracellular store to provide for sustained and repetitive contractions, almost as if there is a fatigue component to the SOCE regulation of contractile force.
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