Another interesting phenomenon observed during these force measurements was that fatigue upon muscle electrode stimulation was reached much faster in control than in silMyoVa transfected animals.
Maximal stimulation was reached after applying 25 μg/kg estrogen.
In the MTS group, motor stimulation was reached in 15 20 s, while in the STS the group sensory stimulation was reached in 5 10 s.
Later, the increase was slow and the maximum level of melatonin secretion, exceeding fivefold the value before stimulation, was reached after two hours of the stimulation.
Significant increases by CXCL12 stimulation were reached only in presence of VCAM-1 (P=0.002), this induction was significantly decreased after treatment with AG490.
Supramaximal stimulation was reached in 13 of 19 TwPdi measurements; in two of 19, supramaximality was not reached; and in the remaining four of 19, insufficient data are available to evaluate supramaximality.
The distance between the 2 stimuli was initially adjusted in steps of 3cm, and then progressively reduced until the minimal distance at which the stimuli were correctly discriminated on 3 consecutive stimulations was reached.
CysLT1-mediated Rac activation was also reported in other types of cells, 31 but this activation was demonstrated to be dependent upon the time of LTD4 stimulation; maximum activation is reached after 15 min, and is mediated through tyrosine phosphorylation and activation of another Rac GEF, Vav2.
When the threshold (or the maximum stimulation time) was reached, stimulation stopped automatically (therefore, we use the term adaptive stimulation), followed by 5 minutes without stimulation.
In some very specific clinical situations, the approach of repeating stimulation until a statistical threshold is reached [15], or using basic t-scores to evaluate the presence or the absence of a particular component [16, 17], has been examined.
