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If stimulation is continued, orgasm usually occurs.
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Photostimulation was delivered using a manual closed-loop protocol: the laser was switched on by the observer as soon as the mouse spontaneously entered the side opposite the one in which he had initially been placed; stimulation was continued until the animal moved to the non-stimulated side, at which point the laser was switched off.
On day 5, the medium containing DMSO was exchanged with regular medium, and the electrical stimulation was continued.
After 1 h, Brefeldin-A (10 μg/ml) was added and stimulation was continued overnight.
The stimulation was continued for 1 min to prevent spontaneous cardiac reversion.
Stimulation was continued briefly when infants were not vigorous, but if there was absent or poor respiratory effort the birth attendants were trained to immediately begin positive pressure ventilation.
To enhance the energy efficiency after 15 h of heating either the input power is increased in incremental stages or thermal stimulation is stopped and extraction is continued with the depressurization technique by pumping out dissociated freshwater from the zone of dissociation within the reservoir at a rate of 144 m3/h with a perforated borehole area of 2 m2 in the reservoir.
From Stimulation Day 8 onwards, the treatment is continued with a daily SC dose of hpHMG (300 IU/day) up to the day of hCG administration.
From stimulation Day 8 onwards, treatment was continued with a daily s.c. dose of FSH (follitropin alfa, follitropin beta or menotropins) until the day of hCG administration (FSH administration on the day of hCG administration was optional).
For experiments using ML171, chloroquine or MG132, the compound was continued during the stimulation, whereas for other treatments, medium containing the compound was replaced by fresh medium according to the experimental requirements.
Following the start of drugs infusion, stimulation at the rate of 4 puffs per hour was continued for 3 h.
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