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Conversely, increasing Ca2+ influx or increasing stimulation frequency accelerates this transition.
A stimulation frequency of 3.6 MHz provided an optimal sound transmission through the polystyrene well plate.
The stimulation frequency was low (20Hz) or high (100Hz).
Moreover, the strength of phase deflections varies with stimulation frequency.
Higher stimulation frequency and non-rhythmical stimulation also produced theta oscillations in the vCA1 (Supplementary Figs. 2 and 3).
Moreover, the model accounts for the dependence of LTP and LTD induction on voltage and presynaptic stimulation frequency.
Note that the stimulation frequency was chosen to mimic the firing rates of natural visual responses in ILSC neurons (Supplementary Fig. 2c,f).
If the τslow component relies on Ca2+-dependent Rf increase, its amplitude should decrease when reducing the stimulation frequency, as the global [Ca2+] is then reduced.
This effect was reduced when lowering external [Ca2+] (Supplementary Fig. 7c) or stimulation frequency (Supplementary Fig. 8a) and increased by addition of TEA (Supplementary Fig. 8c).
The contribution of the slow component increases with stimulation frequency and with release probability and decreases when blocking the docking step with latrunculin.
Blocking SV docking with LatB, decreasing Ca2+ entry and decreasing stimulation frequency all delay the transition from τfast to τslow during an AP train.
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