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This allowed the start of the measurement periods to be closer to the start of stimulation for higher frequencies, which in turn prevented earlier activity that might not have affected the brain's response to stimulation from influencing these measurements.
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The time of stimulation for a high phosphorylation response was chosen from previous experiments [see Additional file 1].
This meant that stimulation artefact was not analysed but fewer data points were analysed for higher stimulation frequencies.
The region which responded earliest to stimulation was then chosen for higher cell resolution imaging and patch clamping (Figure 1a).
This corresponds to the observation that the pain rating after repetitive stimulation was higher for the trigeminal region between the two groups for the different trains applied in our stimulation paradigm.
Crucially, left IPL stimulation slowed responses for high-manipulable items only.
This can engender tolerance, which may manifest as the need for higher levels of stimulation of the reward center to produce a similar "high," and eventually to engaging in characteristic behaviors to avoid withdrawal.
At 4 h post infection a slightly higher uptake rate was found if the cells had been infected with IAV at moi 0.25 but no significant changes were found for higher moi or stimulation with heat-inactivated IAV for 4 h even though the cytokine expression at the same condition was markedly different.
For continuous stimulation, a higher dose led to earlier death and the NF-κB knockout cells died earlier than the wild type ones, especially for low dosage concentrations.
2014 34(12):986-993. 2. Barbanti P, Grazzi L, Egeo G, Padovan AM, Liebler E, Bussone G. Non-invasive vagus nerve stimulation for acute treatment of high-frequency and chronic migraine: an open-label study.
These patients had surgically implanted SPG stimulator devices that used high frequency stimulation for relief of cluster headache.
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