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As stimulation electrode, a patch-clamp pipette was used (1 2 MΩ) to stimulate mossy fibers (mf) in the region of the hilus.
Stimulation electrode locations are the same as the recording electrode locations indicated by dots in d.
The common segment of diode, the doublet, was connected to a stimulation electrode on the head.
(b) A raw voltage trace recorded at an electrode neighbouring a stimulation electrode saturated for about 4 ms (flat line).
The red cross in g indicates the stimulation electrode located near the soma, and arrows indicate the propagation direction.
However, it is difficult to explore the involvement of inflammation and injury with classical electrical kindling, since the stimulation electrode introduces those two factors.
Confirmation of stimulation electrode placement before (dark blue outline), after TTX application (orange outline), and after wash-out (green outline) for recording shown in Fig. 6n.
(a) The duration of a saturated signal occurring after stimuli is plotted versus distance from the stimulation electrode (mean±s.e.m.; N=18 stimulation electrodes from five CMOS-based MEAs).
Our current simulations clearly indicate that medial frontal stimulation can elicit current peaks within the vicinity of ventro-medial prefrontal cortex, in the proximity of the stimulation electrode (see Fig. 1D).
Image of 31-day ALI-CO-mouse co-culture (total organoid age: 79 days) with a stimulation electrode (yellow dashed line) placed on axon tracts projecting from the organoid.
(c) A raw voltage trace recorded at an electrode located 1.46 mm away from a stimulation electrode did not saturate; the signal during stimulation was blanked in software before recording.
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