Sentence examples for stimulation cell from inspiring English sources

Novel strategies employing biomolecules, mechanical stimulation, cell- and gene-therapy have also been pursued.

After stimulation cell free supernatants were collected and IL-23 production was determined by ELISA (eBioscience).

The protease however is speculated to be involved in protease activation, epithelial and keratinocyte differentiation, receptor activation, growth factor stimulation, cell adhesion and matrix degradation [31], [36].

After 1 and 4 hours of stimulation, cell culture medium was removed, each well was washed twice in phosphate-buffered saline, and the plate was frozen at −80°C until ready for nuclear protein extraction.

Five or 10 min after stimulation cell lysates were prepared in Cell Lysis Buffer (Cell Signaling Technology, MA, USA) supplemented with sodium orthovanadate (1 mM), PMSF (1 mM), phosphatase inhibitor cocktail 1 (Sigma-Aldrich, 1∶100) and phosphatase inhibitor cocktail 2 (Sigma-Aldrich, 1∶100).

Previous studies with antigen-specific T cells for the CMV-derived pp65-A2 peptide have shown that upon stimulation, cell division is predominantly restricted to the CD8+CCR7+ population and that CD8+CCR7+ cells are also capable of secreting IFNγ upon antigen-specific stimulation, consistent with our findings.

After PMA stimulation, cells were washed and then stimulated with MSU (100 ug/ml) for 24 h, as described [ 15].

When the cultured plates that are seeded with cells are subjected to biomechanical stimulation, cells are forced to adapt to the deformation of the culture plate.

Prior to stimulation, cells were serum starved for 2 hrs.

At the indicated time points following LPS stimulation, cells were harvested.

Following overnight stimulation, cells were transferred to tubes for flow cytometric acquisition (Dako, USA).