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We treated UM3 cells with 1 μ niclosamide for 2 h and then stimulated them for 15 min with 100 ng/ml tumour necrosis factorα (TNFα).
To assess PDGFR activity, we isolated human FLSs as previously described [ 12], stimulated them for 72 hours with 20 ng/mL PDGF-bb in the presence of small-molecule inhibitors, pulsed them with 1 μCi [H] thymidine (ICN Pharmaceuticals, Costa Mesa, CA, USA) for the final 18 hours of the stimulation, and used a Betaplate scintillation counter (PerkinElmer) to quantify the radioactivity incorporated.
To test for the effect of rapamycin on functional T‐cell exhaustion, we sorted PD‐1+ and PD‐1− T cells from mice fed eRapa or control diet for 6 months (five mice per group), stimulated them for 4 days with anti‐CD3/CD28 antibodies, and assessed proliferation.
Space exploration exposes the next generation of students to the STEM (science, technology, engineering, math) fields, stimulating them to strive for higher education -- a feat our government is so desperately trying to accomplish.
The fatigueability of the muscles was tested by stimulating them at 40 Hz for 250 msec every second.
To address this issue we isolated CD90+ renal fibroblasts from 6 week old mice and stimulated them with agonists for TLR2 (Pam3Cys lipopetide), TLR4 (LPS), TLR9 (CpG-DNA) and with TNF-α.
For instance, participants mentioned that an increase of personal resources (self-efficacy and pro-active behaviour) stimulated them to search for more job resources in their present job.
To prove that K5-expressing cells in HGF-treated spheres originated from luminal progenitors, we isolated Lu-pos cells from Blg-Cre; R26 mouse mammary glands and stimulated them with HGF for 9 10 days.
The volume of PV blood loss stimulated them to ask for blood pressure and pulse readings - against a baseline they were able to detect the deterioration trajectory and thereby deduce hypovolaemic shock.
Their tendency was to right one problem then move on to right the next i.e. low blood pressure stimulated them to call for cannulation and then IV fluids; whilst loss of consciousness might stimulate them to lay the patient flat or elevate the legs, then administer oxygen and or then call for assistance.
This may stimulate them to postpone investments for as long as possible.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com