Moreover, this increased stimulating capacity correlates with an enhanced CD8+ immune response in mice.
This 'lower' stimulating capacity might be the explanation for the lack of 'therapeutic' activity of this TLR4 ligand.
Furthermore, a single substitution of the proline for an serine residue at either p6 or p8 abolished the T cell stimulating capacity.
Thus, E2 can enhance the Th2 stimulating capacity of DC in vitro.
We evaluated BMDC function by antigen-presenting activity and stimulating capacity for cytokine production from responder T cells as previously described (Koike et al. 2009).
To further investigate the effects of the applied loading strategies on HD-derived MoDC, we examined their migratory and T cell stimulating capacity.
Although E2 enhanced the Th2 stimulating capacity of DC in vitro it was found to be dispensable in vivo for the late induction of Th2 immunity in the gender-biased IL-4KO BALB/c mouse model of Trichuris infection.
However, incubation with R848 and CC increased the ability of MoDC to prime naïve T cells in an allogeneic MLR in accordance with the observations for phenotypic maturation: addition of CC after 24 h pre-incubation with R848 enhanced T cell stimulating capacity, but not to the same level as seen for CC alone (Fig. 5c).
Conditoned medium of fibroblasts or desmoid was 5 × concentrated in centriprep tubes YM-10 (Amicon, Millipore Corp., Bedford, MA, USA) and passed through a filter with a 0.22 μm pore size, before testing its invasion-stimulating capacity.
Although it has been described that "naked mRNA" can trigger several pathogen recognition receptors (PRRs) as such providing immune-stimulating capacities,,, - much effort is put into the identification of applicable adjuvants to further activate DCs.
RANKL organizes the cytoskeleton of mature resorptive cells, thereby stimulating their capacity to degrade bone [ 5].
