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This means that MC scholars contemplate, for instance, how liberal democracies should accommodate cultural diversity in their public institutions, while IC scholars reflect on how diverse citizens can be stimulated to enter into dialogue and contact in order to increase social bonds.
About a third report that it changed their lives, making them feel stronger, stimulated to enter education and a career, but the overall endorsement is less enthusiastic.
This is not quiescence because the cells cannot be stimulated to enter the cell cycle again, and the cells do not express senescence markers.
As most current chemotherapeutic strategies target actively cycling cells, dormant and oncogenically mutated stem cells would be immune to such treatment, and could potentially cause leukemic relapse unless they can be pharmacologically stimulated to enter an active state.
Quiescent cells were stimulated to enter the cell cycle by the addition of medium containing 20% FBS.
Cells in the resting (G0) phase are stimulated to enter the active cycle at the first gap (G1) phase.
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Cells were re-stimulated to enter the cell cycle (into G1) by addition of isoleucine-containing MEM or medium containing EGF. Replicating DNA was labeled by either pulsing for 30 minutes with bromodeoxyuridine (BrdU; 15 µM) at the indicated time points, or by continuous labeling with BrdU (20 µM) throughout the experiment.
Computational analyses of our gene expression data suggest that the transcription factor E2F1 may also play a role in hair cell regeneration when many normally quiescent supporting cells are stimulated to re-enter the cell cycle and divide prior to differentiating into new hair cells.
Cells were stimulated to re-enter the cell cycle by the addition of 10% serum for another 36 h.
Following growth arrest, cells were stimulated to re-enter the cell cycle by addition of fresh medium supplemented with 20% FCS.
HCT116 cells were synchronised by serum starvation and the levels of both TS mRNA and protein were studied and compared to the levels of cyclin E as cells were starved or stimulated to re-enter the cell cycle.
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