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We first determined the optimal stimulation duration to observe a difference in intracellular lactate concentration between non-stimulated and stimulated samples.
Significance Analysis of Microarrays (SAM; http://www-stat.stanford.edu/~tibs/SAM) was used to determine which genes were statistically different between stimulated samples and non-stimulated references.
A more informative Y-axis would note that these were stimulated samples, "fusions/24 seconds stimulation".
A response was considered positive when the frequency from stimulated samples was over twice that of non-stimulated cells or those stimulated with unrelated peptides.
Mechanical propertywise, the average value of elastic stiffness increased in the stimulated samples.
The magnitude of impedance at 1 kHz has significantly increased in 24 h post-implant for stimulated samples, which suggests the formation of hemocyte encapsulation.
Similar(23)
Regarding pyruvate, although time effect was less obvious, the same culture duration (overnight+ 24 h) also resulted in measurable difference between stimulated and non-stimulated samples (Fig. 1b).
Figure 2(a) shows typical blots in 2 minutes PE-stimulated samples.
There was variability in different subjects in the magnitude of ileal FGF19 induction after BA stimulation (CDCA-stimulated samples: range 48 939; GCDCA-stimulated samples: range 56 1,065).
Materials and methods: P-selectin expression in non-fixed, whole blood was measured flow cytometrically on non-stimulated and ADP- and TRAP-6-stimulated samples.
Samples tested included antigen-stimulated samples with their negative and positive controls.
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