Suggestions(2)
Exact(9)
The immunofluorescence intensities of CTLs cultured with and without alloantigen stimulation were measured, and the mean fluorescence index (MFI) was calculated as (mean value for stimulated sample – mean value for non-stimulated sample)/mean value for non-stimulated sample.
Unstimulated sample values were subtracted from stimulated sample values.
CMV-specific CD4+ T cells were identified as IFN-γ+ in the stimulated sample.
The majority of the cells in each of these four clusters was derived from the stimulated sample, not the negative control.
A log2 change from the median of measured phospho antibody median fluorescent differences between a stimulated sample and its unstimulated/basal phosphorylation state was calculated.
Figure 2b shows that the forskolin stimulated sample consistently showed a higher fraction of phosphorylated PKA substrate compared to the serum starved sample.
Similar(51)
We first determined the optimal stimulation duration to observe a difference in intracellular lactate concentration between non-stimulated and stimulated samples.
Transcripts for IFNγ, IL-2, IL-4 were statistically significant for differential expression between un-stimulated and stimulated samples using the Tempus™ system (p < 0.05).
Significance Analysis of Microarrays (SAM; http://www-stat.stanford.edu/~tibs/SAM) was used to determine which genes were statistically different between stimulated samples and non-stimulated references.
A more informative Y-axis would note that these were stimulated samples, "fusions/24 seconds stimulation".
A response was considered positive when the frequency from stimulated samples was over twice that of non-stimulated cells or those stimulated with unrelated peptides.
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