Your English writing platform
Free sign upExact(3)
Cells were washed with complete RPMI medium and infected with LVS (MOI of 80 120) or stimulated in parallel with E. coli LPS (10 µg/ml; Sigma-Aldrich #L2360) in triplicate in 0.1 0.2 ml of antibiotic-free medium for 4 h at 37°C, 6% CO2.
As a positive control, macrophages were stimulated in parallel with E. coli LPS. Figure 3A (black bars) shows that peritoneal macrophages from B6 mice respond to LVS infection in vitro by producing increased amounts of TNF, IL-6 and MCP-1 relative to uninfected cells (<20 pg/ml).
After immunisation of DR4-IE transgenic mice with citrullinated human fibrinogen, responses to unmodified native epitopes were stimulated in parallel with responses to citrullinated epitopes.
Similar(57)
Interestingly, Karpas 299 cells with reduced Smac levels demonstrated a significant decrease in NF-κB-responsive genes following CD30 activation, compared with cells transfected with control oligonucleotides and stimulated in parallel through CD30L.
Duplicate PBMC samples (PBMC1 and PBMC2) were stimulated in parallel for 3 and 24 hours to estimate the variability between independent stimulations of the same cell population.
We had the opportunity to test the hypothesis that Gln267 may play a relevant role in the recognition of huCollp261 by the TCR under study, by stimulating in parallel PBMC from patient OE with a peptide encompassing a subdominant epitope of the same protein, namely peptide huCollp289 303 (sequence GKRGARGEOGGVGPI, where O is hydroxyproline, Hyp).
In other words, MICA-129Met variants, which elicit strong NKG2D responses, stimulate in parallel a robust negative feedback signal.
However, staurosporine attenuates the activation of Akt in cultured HEK293 cells stimulated with insulin in parallel with an inhibition of Thr308 phosphorylation, but has no effect on Ser473 phosphorylation (Hill et al, 2001).
In addition, Gic1 and Gic2 were also proposed to be involved in stimulating mitotic exit in parallel with Cla4 by inhibiting GTPase-activating proteins of Tem1, both of which result in Tem1 activation (Höfken and Schiebel, 2004; Tiedje et al., 2008).
Here, we found that nuclear ER β binding protein E2 stimulated T24 cell proliferation in parallel with immediate phosphorylation of ERK.
For unstimulated samples, T cells were mixed with control hamster IgG-linked Dynabeads, and were then cultured in parallel with stimulated samples.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com