Sentence examples for stimulated in duplicate from inspiring English sources

Cells were stimulated in duplicate with medium only, PHA (Sigma-Aldrich, Gillingham, UK) at 10 µg/ml or specific peptide at 10−5 µM in a final volume of 120 µl for 1 hour at 37°C in 5% CO2. 30 µl of filtered FCS was then added to each well and the plates were incubated at 37°C in 5% CO2 for 16 20 hours.

Peripheral blood mononuclear cells (PBMC) were isolated within 12 h after venipuncture and stimulated in duplicate as described elsewhere [ 14].

PBMC (2 × 10/well) were pre-stimulated in duplicate in 96-well plates with culture medium containing 3 μg/ml HuD 9-mers, HuDmix, CMV-pp65, PHA, or no antigen for 1.5 h at 37°C and 5% CO2 [ 10].

Duplicate PBMC samples (PBMC1 and PBMC2) were stimulated in parallel for 3 and 24 hours to estimate the variability between independent stimulations of the same cell population.

Control or stimulated wells were established in duplicate and the cell culture was incubated for 24 h at 37 °C in an atmosphere of 5%% CO2 in air.

APCs (10 cells/mL) were seeded in 96-well plates in duplicate and stimulated for 24 h at 37°C and 5% CO2 with 10  μg/mL of supernatants containing saline vehicle or induced-excreted proteins from infected or uninfected mites.

For mRNA isolation, Kupffer cells (in duplicate) were stimulated with 10 μg/ml LPS, and mRNA was obtained 2, 4, and 6 h from LPS-treated as well as from control cultures.

20 Briefly, viable PBMC (200 000/well) plated in duplicate were stimulated for 18 h with peptide pools A-M (3 μg/ml), cytomegalovirus (CMV) lysate (0.05 μg/ml, Chiron), influenza, Epstein-Barr virus (EBV) and CMV FECCD8CD8 epitopes in a single pool (3 μg/ml BEI resources).

Stimulations were performed in duplicate.

In duplicate experiments, mastoparan-7 stimulated PtdBut formation 1.33/1.7 times over basal in PNT2C2 cells, 2.8/2.6 times in P4E6 cells and 4.7/3.6 times in PC3 cells.

When the average number of spot-forming cells in duplicate wells of stimulated cultures exceeded that in controls by 10 spot-forming cells and, at the same time was above 34 spot-forming cells/million PBMC the responses were scored as positive [ 26].